Jeffrey Tambor is facing a second round of sexual misconduct allegations, this time from his Transparent co-star Trace Lysette. The actress, according to The Hollywood Reporter, alleges that the star made lewd remarks toward her and tried to touch her inappropriately on the set of their hit Amazon series. This is the second sexual misconduct allegation that has been made against Tambor, who was previously accused of inappropriate behavior by Van Barnes, his personal assistant on the show.

Lysette, who is trans, plays Shea on the show, a trans woman who befriends Maura Pfefferman, the older trans character played by Tambor. She claims that in Season 2, while they were filming a bonding scene between their characters, Tambor made lascivious remarks toward her when she emerged from wardrobe. “My God, Trace. I want to attack you sexually,” Lysette recalls him saying. Alexandra Billings, the third actress in the scene, told T.H.R. that she heard the remarks. They both “laughed it off because it was so absurd,” Lysette says.

Later, when Tambor was in his Maura costume, he allegedly approached Lysette inappropriately. “He came in close, put his bare feet on top of mine so I could not move, leaned his body against me, and began quick, discreet thrusts back and forth against my body. I felt his penis on my hip through his thin pajamas,” Lysette says.

Per T.H.R., Lysette says she pushed Tambor away and rolled her eyes. Billings was not present. There were crew members around, “but they were focused on their jobs. It was discreet. If you were behind Jeffrey you might have thought he was giving me a hug.”

Lysette says that at the beginning of their work relationship, Tambor was friendly enough, even sticking up for her one day when she kept missing her mark. But his behavior slowly changed, she says. She alleges that when she saw him at red-carpet events, she would “go to hug him and kiss him on the cheek—and it would land on my lips. And I was like, ‘O.K. What was that about?’”

She alleges that after Tambor set up a meeting between Lysette and his manager, she thanked Tambor. Tambor allegedly responded, “Well, yeah Trace. I really believe in you. And you know what? I don’t even want to have sex with you.’ And then he just looked at me as if he very much indeed wanted to have sex with me. It was an up-and-down look. I rolled my eyes and went to my trailer and thought, ‘This man is something else.’ ”

The accusations arrive not long after Barnes's allegations, which were made in a private Facebook post about an unnamed employer obtained by T.H.R. Barnes claimed that the employer made sexually charged remakes, including saying that “I should be sleeping with him if I want a Hollywood industry appropriate pay.” In addition, she said her employer would pat her on the bottom, play pornography at loud volumes, and call her “useless.” Amazon launched an investigation into Tambor once the claims went viral; the actor responded to the allegation with an initial statement in which he called Barnes “a former disgruntled assistant” and said, “I adamantly and vehemently reject and deny any and all implication and allegation that I have ever engaged in any improper behavior toward this person or any other person I have ever worked with.”

Lysette was Barnes’s roommate when Barnes worked as Tambor’s assistant. “She would come home wrecked and stressed. I saw him break her down. She for the most part kept it to herself. She was just like, ‘Girl, he is a piece of work. If you only knew what I’m going through.’ But I knew,” she told T.H.R. The actress says both she and Barnes went to Amazon with their allegations, but no action was taken.

“Despite having made no public statements to date, the disclosure of Van Barnes's identity by the press has already led to Ms. Barnes receiving numerous threats and harassment online,” Barnes’s lawyer, Alana Chazan, told T.H.R. “Ms. Barnes is cooperating with the investigation being conducted by Amazon and will not be making a statement while the investigation is pending.”

GLAAD issued a statement in support of Lysette: “Trace Lysette is a gifted actress and a trailblazer for the inclusion of transgender women in Hollywood. For too long transgender women have been forced to hide stories of harassment and abuse in the workplace, and Trace has taken a powerful stand in calling for an industry where all women can work in safe environments. The show Transparent is bigger than one person and is home to some of the most talented trans people in Hollywood – both in front of the camera and behind. GLAAD stands with Trace in her hope that the inappropriate situations she and others endured on set will be remedied, and that future seasons will focus on more of the many brilliant characters that audiences love and care about.”

Tambor, for his part, has responded to the latest allegations with a lengthy statement, apologizing for his past behavior but reiterating that he is “not a predator.”

“For the past four years, I’ve had the huge privilege—and huge responsibility—of playing Maura Pfefferman, a transgender woman, in a show that I know has had an enormous, positive impact on a community that has been too long dismissed and misunderstood. Now I find myself accused of behavior that any civilized person would condemn unreservedly. I know I haven’t always been the easiest person to work with. I can be volatile and ill-tempered, and too often I express my opinions harshly and without tact. But I have never been a predator— ever. I am deeply sorry if any action of mine was ever misinterpreted by anyone as being sexually aggressive or if I ever offended or hurt anyone. But the fact is, for all my flaws, I am not a predator and the idea that someone might see me in that way is more distressing than I can express.”